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. 2003 Dec;133(4):1768–1778. doi: 10.1104/pp.103.026013

Figure 2.

Figure 2.

Western- and northern-blot analysis of Fd expression. A, Leaf extracts of potato plants (100 μg of soluble protein per lane) were subjected to SDS-PAGE and transferred to nitrocellulose. Immunodetection was performed with an antiserum raised against spinach Fd I. Samples were prepared from wild type (WT) and from transgenic lines A62, A93, A99, A100, and A150. B, Aliquots of 100 μg of soluble protein from tubers of the same plants as in A were probed with the potato Fd I antiserum. C, Abundance of fed 1 mRNA in potato leaves was analyzed with a northern blot. Total leaf mRNA (30 μg lane-1) was subjected to denaturating agarose gel electrophoresis, blotted to nylon membranes, and probed with randomly primed [32P]-labeled fed1 cDNA fragments from potato.