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. 2003 Dec;133(4):1779–1790. doi: 10.1104/pp.103.030742

Figure 1.

Figure 1.

Root-vascular tissue-specific XSP30 expression. A, Root-specific expression of XSP30. Total RNA (10 μg lane-1) extracted from aboveground organs or roots of 30-d-old cucumber plants was subjected to RNA gel-blot analysis. The transcripts were probed using a full-length XSP30 cDNA and ubiquitin fragments as probes. The ubiquitin cDNA was isolated from cucumber roots, where it is expressed constitutively. B through D, β-Glucuronidase (GUS) activity in PXSP30::GUS-transgenic hairy roots. GUS activity was observed in the central cylinder of mature roots, but not in the root tip shown in B. GUS-stained transgenic roots were embedded in Technovit 7100 (Kulzer and Co., Werheim, Germany), and thin serial sections were stained with toluidine blue (C) or left un-stained (D). The central cylinder is shown. Scale bars correspond to 100 μm. The arrow and arrowhead indicate GUS staining in the xylem parenchyma and pericycle cells, respectively. E, Seedling development-dependent expression of XSP30 in roots. Total RNA (10 μg lane-1) extracted at dusk from roots of seedlings at 4, 6, 8, 10, 12, 14, 16, and 18 d after sowing were subjected to RNA gel-blot analysis. The transcripts were probed using a full-length XSP30 cDNA and a ubiquitin fragment as probes.