Table I.
Identified proteins of the apoplast of cowpea after separation by BN-/SDS-PAGE
| Noa | Ionb | Sequencec | Identified Proteind | Accession No. | Matching Sequence | ple | Mre | Reference |
|---|---|---|---|---|---|---|---|---|
| 1 | 992.38 | QEVVDLYK | *1,3-β-glucanase (soybean [Glycine max]) | T05957 | QEVVDLYK | 4.27 | 26,074.11 | |
| 1,074 | HFGLFNPDK | 1,3-β-glucosidase (barley [Hordeum vulgare]) | AAA32960 | HFGLFNPDK | 8.46 | 32,583.82 | Xu et al. (1992) | |
| 1,266.36 | VNYYTEYCR | Chitinase class 4 (cowpea) | S57476 | VNYYTEYCR | 4.25 | 26,876.36 | ||
| 4 | 762.32 | MGASLLR | Peroxidase (wheat [Triticum aestivum]) | AAM76682 | MGASLLR | 8.89 | 32,425.66 | |
| Peroxidase POC1 (rice [Oryza sativa]) | AAF65464 | MGASLLR | 6.40 | 32,461.60 | ||||
| 5 | 978 | GSDGSVLGCK | *Thaumatin-like protein 1 precursor (sand pear [Pyrus pyrifolia]) | O80327 | GSDGSVIGCK | 5.07 | 25,308.31 | Sassa and Hirano (1998) |
| 1,693 | SACLAMGDDQYCCK | *Thaumatin-like protein 1 precursor (sand pear) | O80327 | SACLALNQPQYCC | 5.07 | 25,308.31 | Sassa and Hirano (1998) | |
| 1,069.42 | TGCNFDGDGK | Thaumatin-like protein PR-5a (chickpea [Cicer arietinum]) | CAA09229 | TGCNFDGSG | 5.51 | 18,732.00 | ||
| Pathogenesis-related protein 5 precursor (Arabidopsis) | JQ1695 | TGCNFDASGNG | 4.75 | 25,252.31 | Uknes et al. (1992) | |||
| 898 | LPSPWSGR | Thaumatine-like protein (apple [Malus × domestica]) | AAM12886 | PSPWSGR | 4.60 | 22,107.61 | Venisse et al. (2002) | |
| 7 | 1,095 | YGGVMLWDR | *Chitinase (EC 3.2.1.14) class III, acidic (soybean) | T05187 | YGGVMLWDR | 4.96 | 37,110.79 | Yeboah et al. (1998) |
| 1,909 | CNPSLNCNVFSDLQK | *Chitinase (EC 3.2.1.14) class III, acidic (cowpea) | S57468 | CNPSINNCNVFSD | 4.81 | 26,326.12 | ||
| 966 | VGFGSAASGSK | Endosperm-specific protein-like protein (Arabidopsis) | AAM66074 | VGFGSAASGSK | 5.43 | 43,060.81 | Haas et al. (2002) | |
| Fasciclin-like arabinogalactan-protein (Arabidopsis) | NP_566043 | VGFGSAASGSK | 5.43 | 43,074.83 | ||||
| 1,434 | QQFPLAVXXXDK | Fasciclin-like arabinogalactan-protein (FLA6; Arabidopsis) | NP_565475 | QQFPLAV | 5.51 | 26,506.10 | ||
| 8 | 1,454 | AVSAYCSTYDADK | Hevein-like protein precursor (Arabidopsis) | P43082 | AVSAYCSTWDADK | 7.89 | 22,936.63 | Potter et al. (1993) |
| Wound-induced protein (clone TAB7; tomato [Lycopersicon esculentum]) | T07729 | AVSAYCSTWDANK | 8.38 | 21,607.17 | Harris et al. (1997) | |||
| *Putative pathogen-induced protein (tomato) | CAC81819 | AVSAYCSTWDANK | 6.62 | 14,960.57 | ||||
| 10 | 1,636 | YDYGSNTCVGGECR | PR-1 protein (wine grape [Vitis vinifera]) | CAA05868 | YDYNSNSCVGGEC | 6.05 | 11,078.28 | |
| 1,013 | LWVDEKP | Pathogenesis-related protein 1-1a (cucumber [Cucumis sativus]) | AAL84768 | LWVDEKP | 6.92 | 15,655.49 | ||
| 1,453 | YGENLAGSSGDLSG | *Pathogenesis-related protein 1 (European white birch [Betula pendula]) | AAF62171 | YGENLAASSGDLSG | 5.99 | 10,819.80 |
a The nos. correspond to the nos. given in Figure 6. b Ion precursor (mass/charge unit). c Amino acid sequence as determined by ESI-MS/MS. Due to similar molecular properties, Leu and Ile cannot be distinguished. L represents both. d Proteins were identified by http://www.ncbi.nlm.nih.gov/BLAST/. Stars indicate correspondences to Table II. e pl and molecular mass were calculated by http://www.expasy.org/.