Table II.
Identified proteins of the apoplast of cowpea separated by IEF/SDS-PAGE
| No.a | Ionb | Sequencec | Identified Proteind | Accession No. | Matching Sequence | ple | Mre | Reference |
|---|---|---|---|---|---|---|---|---|
| 2 | 1,449.48 | SAYCSTYDA | *Putative pathogen-induced protein (tomato) | CAC81819 | SAYCSTWDA | 6.62 | 14,960.57 | |
| Wound-induced protein 2 percursor (potato [Solanum tuberosum]) | S04927 | SAYCSTWDA | 7.37 | 22,498.23 | Stanford et al. (1989) | |||
| Hevein-like protein precursor (Arabidopsis) | P43082 | SAYCSTWDA | 7.89 | 22,936.63 | Potter et al. (1993) | |||
| 3 | 1,449.57 | GLSAYCRQ | ? | |||||
| 8 | 1,652.52 | NRDYGSNTCVG | Pathogenesis-related protein (barley) | CAA52893 | DYGSNTCAG | 8.19 | 17,439.62 | Bryngelson et al. (1994) |
| 2,794.21 | VAA(M/F)A(Q/K)NYANQR | Pathogenesis-related protein (Tobacco [Nicotiana tabacum]) | S22531 | AAFAQNYANQR | 7.62 | 20,120.38 | Eyal et al. (1992) | |
| 1,714.56 | ESPEADYLLNEHNAAR | Pathogenesis-related protein (tobacco) | S22531 | SPQ-DYL-NPHNAAR | 7.62 | 20,120.38 | Eyal et al. (1992) | |
| 9 | 1,637.52 | YGENLAGSSGDLSGK | *Pathogenesis-related protein (birch) | AAF62171 | YGENLAASSG-DLSG | 5.99 | 10,819.8 | |
| 10 | 1,636.52 | QNYGSNTCVGWCR | Pathogenesis-related protein precursor (barley) | S52627 | NYGSNTC | 9.08 | 17,678.86 | Mouradov et al. (1994) |
| 13 | 1,722.72 | SSWNQWTS | *Chitinase class III, acidic (cowpea) | S57468 | SSWNQWTS | 4.81 | 26,326.12 | |
| YGGVMLDSDR | *Chitinase class III, acidic (soybean) | T05187 | YGGVML-WDR | 4.96 | 37,110.79 | Yeboah et al. (1998) | ||
| 14 | 1,721.74 | (LS)LSSWNQWTSSQAK | Chitinase (adzuki bean [Vigna angularis]) | P29024 | SSWNQWTSSQAK | 4.9 | 31,701.55 | Ishige et al. (1993) |
| 16 | 1,721.74 | YGGV(F/M)LWDR | Chitinase class III, acidic (soybean) | T05187 | YGGVMLWDR | 4.96 | 37,110.79 | Yeboah et al. (1998) |
| 945.46 | AL(N/D)GLSSQR | Chitinase class III (African yam-bean [Sphenostylis stenocarpa]) | AAD27874 | ALNDLSSQR | 4.08 | 31,392.93 | Colucci et al. (1999) | |
| Putative peroxidase (rice) | AAL34128 | LDGLSS | 4.65 | 33,956.4 | ||||
| 1,222.46 | EGTLAETCNTK | Chitinase class III (African yam-bean) | AAD27874 | EGTLADTCNT | 4.08 | 31,392.93 | Colucci et al. (1999) | |
| 18 | 1,060.57 | HFGLFTPDK | 1,3-β-glucanase (maize [Zea mays]) | T02088 | HFGLFNPDK | 4.56 | 34,893.92 | Wu et al. (1994) |
| 1,559.79 | TQLNAACPWLKMK | Peroxidase, cationic (Townsville stylo [Stylosanthes humilis]) | AAB67737 | TQLEAACP | 7.06 | 33,781.18 | Stines et al. (1996) | |
| 978.45 | (GS)DGSVLGCK | *Thaumatin-like protein 1 precursor (sand pear) | O80327 | DGSVIGCK | 5.07 | 25,308.31 | Sassa and Hirano (1998) | |
| 20 | 992.54 | KEVVDLYK | *1,3-β-glucanase (soybean) | T05957 | EVVDLYK | 4.27 | 26,074.11 | |
| 974.57 | AAPVVDLYK | Glucan endo-1,3-β-d-glucosidase (chickpea) | CAA10167 | VVDLYK | 5.49 | 35,595.06 | ||
| 1,385.77 | QRGLFNPDKSPK | Glucan endo-1,3-β-d-glucosidase (Chickpea) | CAA10167 | GLFNPDKSPK | 5.49 | 35,595.06 |
a The nos. correspond to the nos. given in Figure 7. b Ion precursor (mass/charge unit). c Amino acid sequence as determined by ESI-MS/MS. d Due to similar molecular properties, Leu and Ile cannot be distinguished. L represents both. e Proteins were identified by http://www.ncbi.nlm.nih.gov/BLAST/. Stars indicate correspondences to Table I. Isoelectric point and molecular mass were calculated by http://www.expasy.org/.