Figure 1.

Fluorescence differential display screen for auxin-inducible transcripts. A, Scheme of the experiment: 1, coleoptiles were grown for 6 d in darkness; 2, coleoptile segments were excised; and 3, segments were predepleted in water for 1 h and then incubated for 1 more h in IAA or in water. B, A part of an FDD gel image showing the CYP87A3 expression pattern in WT and the mutant Yin-Yang. Total RNA was isolated from coleoptile segments incubated for 1 h either in distilled water (lanes 1 and 3) or in 5 μm IAA (lanes 2 and 4).