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Kinetics of CYP87A3 expression in response to exogenous IAA. Total RNA (15 μg per lane) was isolated from coleoptile segments that either had been excised directly from seedlings grown for 6 d in darkness (lane 9) or that after excision had been incubated in water (lanes 1-4) or in 5 μm IAA (lanes 5-8), respectively. The same membrane was hybridized with probes specific to CYP87A3 and 28S rRNA (loading control).
