Figure 6.

CYP87A3 transcripts are elevated after cycloheximide treatment. Total RNA (15 μg per lane) was isolated from coleoptiles that have been treated with cycloheximide and/or auxin. When coleoptiles were treated by cycloheximide in combination with auxin (lane 7), cycloheximide was applied 30 min before IAA to ensure inhibition of protein synthesis before the exogenous auxin could enter the cell. The working concentrations were 5 μm for IAA and 70 μm for cycloheximide. For details, refer to the legend of Figure 4. CHX, cycloheximide.