Table I.
Primers used in reverse transcription (RT)-PCR analyses and cloning
Primer Name and Specificity | Sequence |
---|---|
RT-PCR | |
LePPCK1, 5′ | 5′-TCA AAT TTG CGA AGA AAT CG-3′ |
LePPCK1, 3′ | 5′-CCT TCT CCT TCT CTC TTC CAC A-3′ |
LePPCK2, 5′ | 5′-GAG CTA TTC GCC GTC AAG TC-3′ |
LePPCK2, 3′ | 5′-AAA TCA GCC AAT TTC AGT TCG-3′ |
Le Actin 52, 5′ | 5′-GAT GCC TAT GTT GGT GAC GA-3′ |
Le Actin 52, 3′ | 5′-ATC CTC CGA TCC AGA CAC TG-3′ |
StPPCK2, 5′ | 5′-GAG TCA TTC GCC GTC AAG TC-3′ |
StPPCK2, 3′ | 5′-AAA TCA GCC AAT TTC AGC TCG-3′ |
Cloning | |
LePPCK2, 5′ FL | 5′-AGG AAT TCG GCA CGA GAA A-3′ |
LePPCK2, 3′ FL | 5′-GAC CAG AGT ATA TTG GTG CCT GT-3′ |
StPPCK1, 5′ | 5′-ATG TTC CAA ACC TTG AAA AA-3′ |
StPPCK1, 3′ | 5′-TCA GTT TAG ATC AGC CAT TG-3′ |