Oxygen affects key enzymatic activities of carbohydrate and lipid metabolism. Siliques (30 DAF) were exposed to 0%, 4%, 12%, 21%, 30%, and 60% (v/v) O2 for 2 h, then rapidly frozen in liquid N2, and seeds were separated from the silique wall under liquid N2 to analyze enzyme activities: A, SuSy; B, invertase; C, ACCase; D, G3PAT; and E, DAGAT. Results are means ± se (n = 3 separate siliques from different plants). Error bars are not shown when they are smaller than the symbol.