Table I.
Sequences of the gene-specific primers used in real-time PCR assays of gene expression in Synechococcus sp. PCC7942
| Gene | GenBank Accession No. | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|---|
| ccmK | M96929 | TTTTTAAAGGACGTGGACTC | GCAGAGACAGAAGCTTGAAC |
| ccmL | M96929 | TTCTTGGTTGTTCAGTTCTTG | TATCGATGATGGCAATGAC |
| ccmM | M96929 | AGTCGCGATTACTCACAAAG | AGGAATCTCTACGTCTTGGAC |
| ccmN | M30808 | AGAGTATTGTCGGTCGTCAG | ACTGTTCCTTGCCGTAGAC |
| ccmO | M30808 | TAGACGACTTGATGGACAGC | GTCGCTCTTCTGCTAAAAGTC |
| chpX (cupB) | AY029339 | TGCCTACTCTCGTAATCTCTG | CATCGAGAAAGGTATCTAATCC |
| chpY (cupA) | AY029338 | GCTTTACCTGGCTCTATGAAG | AGCAAGTAGGGTGGTAAGAAG |
| cmpA | M32999 | TCGCAACTTCTTTAACGTG | TTGTAGGGATAGGAGACACTG |
| cmpB | D26358 | TCGAAAAGCAAGTACCTAATG | AACAGCACCGATATAAATCAC |
| cmpC | D26358 | AGGATTGATCATGAGTTTATTTG | CAACTCTAGACCAGCAATCAG |
| cmpD | D26358 | CAGAGGACTTGTGTCATGC | AAGATTGACTCCATCCAAAAC |
| cmpR | AB047379 | ATTACTGTGCAACCATTTCTC | CGTTCAAATTGAGGTTATGAG |
| cynA | AF001333 | CAATTTACCTGACGACTGAAG | CGTATTTCTGCTTTTGAGATG |
| dc13 | U62616 | CTCAGAAATTCCAAGTGGTC | AGATTGGTCAGTTCACGTTC |
| dc14 (ictB) | U62616 | CAGCTTATGTCTGATCCTCAC | AAGTTGTTGCTGCTGTCTTC |
| glnB | AF079137 | GAGTTCCCTTGAAGAAGATTG | AAAACTCAACCGTGTATTCC |
| icfA (ccaA) | M77095 | CTGTTCATTACCTGCTCAGAC | CAACATGCTCAATGTTCAAAG |
| ndhD3 | AY029338 | AGTTTGTTGATGGGCTTTG | CCAGAAAAATCAGGCTGTAG |
| ndhD4 | AY029339 | GCACTCTTCTTCCAGTTCG | AATGATCAGGAAGGTTAGGAG |
| ndhF1 | AY017305 | TACATGTTCCGCATCTACTTC | CCAGTAGACCGATCAGAGC |
| ndhF3 | AY029338 | CTACACGCCAATCCTTTATC | GCATCAGGAAGAAGACACTG |
| ndhF4 | AY029339 | CAATGGCAGCTGTTAGTCAC | CGATCGATGTAGAAGTCGTAG |
| ORF133 | AY029338 | CAACTAGCTCGAATTCTCAAG | GTTATCACAGGCAATATCAGC |
| PEP carboxylase (ppc) | M11198 | GCCTCAAGCTCTCCTATATTC | GAATCAGCTTGAGATCTTCG |
| petB | U33285 | TTGTTTTCTCATTCAGTTTGC | GAAACCACCAGTCAGATAAAC |
| petE | U20147 | TCGGTAGCTTCTTCCTCTC | GAAGGTAGCTTCAAAGGTTTC |
| pmta | AF428100 | AAAGACCTTACCAAGATGAGC | ACCGAAGCTACTGATCAAGAC |
| psaE | M99432 | GAGGAAGCATCAGTTATGG | AGCCGTTGTAGTTCACTTTC |
| psbAI | X04616 | CCTTTACAACCTCAAGATCG | AATGAACGCAACGATGAAGC |
| psbAII | X04616 | TAGTTCAACTAAGGACTCATC | GATGAACGCAACGATGAAGC |
| psbAIII | X04616 | ATTCACTGGACTCAAAACATC | GATGAACGCAACGATGAAGC |
| psbDI | M20814 | AGTACGGCAAGAGGTTTTAG | CTAAATACGCACAGGGAAAC |
| psbDII | M20815 | CAAATATCTGGAGATTGCTAAG | CTAAATACGCACAGGGAAAC |
| rbcL | J01536 | CTATTACACCCCCGATTACAC | GTAGCACTTGCCTTTGTACC |
| rbcR | AY017305 | AAGGTGATTGACCAAGTGC | GGTTGTAGATTACCGATAGGG |
| sbtA | unpublished datab | AATATGCTCAGCAAGAGTCTG | CTTTACGCAGCTCACTAATTC |
a Putative membrane transporter. b See “Materials and Methods.”