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Subcellular localization of GAPN activity in endosperm and shoot cells of wheat
| Enzyme
|
Activity
|
||
|---|---|---|---|
| Total | in Supernatant | in Pellet | |
| units g fresh wt−1a | % | ||
| Endosperm | |||
| GAPC | 75 | 95 | 2.0 |
| Soluble starch synthase | 4.7 | 3.2 | 98 |
| ADPGlc pyrophosphorylase | 5.2 | 5.1 | 92 |
| GAPN | 3.0 | 1.5 | 88 |
| Shoot | |||
| GAPC | 130.0 | 98 | n.d.b |
| Glc 6-P dehydrogenase | 43 | 10 | 88 |
| Aldolase | 17.8 | 92 | 1.2 |
| GAPN | 6.0 | 95 | n.d. |
a Units of activity per gram of fresh wt. b n.d., Not detected.
