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. 2010 Sep 10;18(1):42–52. doi: 10.1038/cgt.2010.49

Figure 5.

Figure 5

Apoptosis induction in GLV-1h68-infected tumor cells on prodrug treatment. Western blot analysis using specific antibodies against cleaved poly (ADP-ribose) polymerase (PARP), full-length (FL) caspase-3, cleaved (CL) caspase-3, full-length caspase-9 and β-actin. Samples were taken from infected or mock-infected GI-101A cells treated (+) or untreated (−) with 10 n prodrug. Expression of the apoptosis indicator PARP was significantly higher in GLV-1h68-infected cells treated with prodrug, compared with cells treated with vaccinia virus alone or with GLV-1h43 and prodrug. Uninfected control cells showed almost no expression of PARP. Furthermore, approximately no full-length caspase-3 or caspase-9 could be observed in GLV-1h68-infected cells treated with prodrug, whereas both proteins were present in the other samples. Cleaved caspase-3 was detectable only in those samples retrieved after combined treatment with GLV-1h68 and prodrug. Equal loading of protein was confirmed by detection of β-actin. PBS, phosphate-buffered saline.