Table 2.
Expression of Genes Encoding Select Prominent Constituents in Corneocyte Cross-Linked Proteomea
| Tissue/Marker | DSP | JUP | PPL | AHNAK | ANXA1 | SEBP1 | EVPL | PKP1 | FLG2 | KPRP | LRRC15 | VSIG8 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Brain | 33.62 | 30.37 | 32.14 | 30.60 | 28.50 | 29.83 | ND | 35.50 | ND | ND | ND | ND |
| Placenta | 24.73 | 23.71 | 27.31 | 21.14 | 19.71 | 28.28 | 26.83 | 28.25 | ND | ND | 29.50 | 35.88 |
| Lung | 29.87 | 25.72 | 28.77 | 22.18 | 21.39 | 25.77 | 26.86 | 29.86 | ND | ND | 38.60 | 35.28 |
| Liver | 27.03 | 24.58 | 27.63 | 22.69 | 24.64 | 22.89 | 34.62 | ND | ND | ND | ND | 38.84 |
| Heart | 24.84 | 24.63 | 31.00 | 22.65 | 24.88 | 27.32 | ND | 35.39 | ND | ND | ND | ND |
| Skeletal Muscle | 34.92 | 26.67 | 32.49 | 23.94 | 25.80 | 26.94 | ND | 33.29 | ND | ND | ND | ND |
| Kidney | 26.78 | 23.98 | 28.49 | 22.28 | 24.66 | 24.38 | 25.39 | 32.17 | ND | ND | ND | 33.27 |
| Pancreas | 23.60 | 23.77 | 26.15 | 21.34 | 23.88 | 25.00 | 24.87 | 32.16 | ND | ND | ND | ND |
| Prostate | 27.28 | 25.64 | 27.75 | 23.92 | 23.22 | 25.83 | 27.02 | 27.92 | ND | ND | ND | ND |
| Testis | 30.81 | 27.60 | 29.99 | 24.86 | 27.00 | 28.35 | 30.32 | 34.59 | 36.00 | ND | ND | 35.41 |
| Ovary | 28.76 | 25.62 | 28.47 | 21.04 | 23.69 | 25.22 | 31.14 | 34.40 | ND | ND | ND | 35.80 |
| Small Intestine | 31.04 | 27.30 | 29.22 | 22.67 | 24.43 | 25.77 | 27.97 | 33.59 | ND | ND | ND | ND |
| Colon | 27.96 | 26.91 | 29.47 | 23.41 | 25.27 | 23.75 | 28.25 | 35.50 | ND | ND | ND | ND |
| Thymus | 27.38 | 26.63 | 28.26 | 23.59 | 24.31 | 27.55 | 29.62 | 27.28 | 32.81 | ND | ND | 34.22 |
| Spleen | 28.83 | 27.02 | 34.02 | 22.76 | 24.37 | 25.47 | 37.34 | ND | ND | ND | ND | 38.06 |
| Leukocyte | 35.50 | 27.80 | ND | 21.87 | 22.49 | 33.50 | 34.91 | ND | ND | ND | ND | 38.35 |
| Epidermis 1 | 19.96 | 20.30 | 23.06 | 21.96 | 24.94 | 28.73 | 26.37 | 21.88 | 20.99 | 23.92 | 33.89 | ND |
| Epidermis 2 | 19.73 | 20.01 | 23.10 | 21.79 | 24.28 | 29.00 | 25.36 | 21.60 | 20.17 | 24.63 | 34.41 | ND |
| Culture 1 | 17.96 | 18.08 | 18.93 | 19.62 | 17.36 | 29.93 | 22.96 | 19.89 | 19.99 | 17.75 | 34.97 | ND |
| Culture 2 | 17.86 | 17.98 | 19.17 | 18.92 | 17.92 | 28.98 | 22.45 | 19.24 | 20.48 | 19.83 | 37.11 | ND |
a
Ct values for β-actin were 21.4 ± 1.4 in the commercial panel, 23.5 ± 0.1 for the skin samples and 19.8 ± 0.03 for the cultures. ND, Not detected (Ct ≥40)