Fig. 4.

mec-10(tm1552) dominance tests assayed by calcium imaging. A: stimulus positions for imaging experiments. Animals expressing cameleon in touch neurons were given a 1-s gentle (buzz) stimulus at the indicated position, as described in methods. B–D: averaged calcium responses of wild-type (B), mec-10(tm1552)/+ (C), mec-10(tm1552) (D, red trace), and mec-10(tm1552); ljEx228[pmec-4::mec-10; pmyo-2::GFP] (D, green trace) animals. Each trace represents the average percentage change in R/R₀, where R is the fluorescence emission ratio at a given time point and R₀ is its initial value. The number of individual recordings averaged for each trace were n = 13, 13, 13, and 13 (wild-type, positions 3, 2, 1, and −1, respectively); n = 13, 13, 13, and 13 [+/mec-10(tm1552) heterozygous positions 3, 2, 1, and −1, respectively]; and n = 13, 13, 13, and 13 [mec-10(tm1552) homozygous, positions 3, 2, 1, and −1, respectively]. Gray shading indicates SE of the mean response. Scale bars are indicated in the top right. The green bar indicates the time of the stimulus. E: scatterplot of peak calcium responses for each genotype. Red lines indicate the mean response at each of the 4 stimulus points; error bars indicate SE. Every other line indicates the response for a single animal. Statistical significance (***P < 0.0005; **P < 0.005) is according to the Mann–Whitney rank-sum test (asterisks at stimulus points indicate comparisons to wild-type; asterisks on bars indicate comparisons to the rescue line). Half the animals were stimulated from anterior to posterior and half from posterior to anterior.