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. 2010 Sep 13;29(48):6343–6356. doi: 10.1038/onc.2010.366

Figure 5.

Figure 5

Inhibition of ILK activity or expression regulates TWIST expression. (a) SKBR3 cells were treated with 42 μ QLT0267, Neg siRNA or ILK siRNA. Subsequently, cells were lysed, protein was isolated and then separated on a 10% SDS–PAGE gel. Resulting western blots were probed for ILK, TWIST and β-actin. TWIST protein is reduced by 98% in SKBR3 cells treated with QLT0267 or nucleofected with ILK siRNA when compared with controls (untreated or Neg siRNA, respectively). (b) SKBR3 cells were transiently nucleofected with Control or 4 μg TWIST siRNA for 96 h. Subsequently, cells were lysed, protein was isolated from samples, separated on a 10% SDS–PAGE gel and probed for Her2/neu, YB-1, TWIST and β-actin. Silencing of TWIST is seen at 96 h. With a 40% silencing of TWIST, YB-1 is decreased by 47% and Her2/neu total protein is reduced by 70%.