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. 2010 Aug 30;29(48):6390–6401. doi: 10.1038/onc.2010.361

Figure 2.

Figure 2

CpG island methylation of T-UCRs in colon cancer cell lines and normal tissues. (a) Bisulfite genomic sequencing of the associated CpG islands for the T-UCRs Uc.160+, Uc.283+A and Uc.346+ in the colorectal cancer cell lines HCT-116 and DKO, normal colon (NC) and normal lymphocytes (NL). CpG dinucleotides are represented as short vertical lines and the location of the T-UCR probe from the expression microarray is represented as a black arrowhead. The locations of the bisulfite genomic sequencing primers are indicated by black arrows. Eight single clones are represented for each sample. Presence of a methylated or unmethylated cytosine is indicated by a black or a white square, respectively. For Uc.283+A and Uc.346+, two regions within the CpG island were analyzed. The transcription start sites identified by RACE are represented by vertical black arrows. (b) Methylation-specific PCR analyses for Uc.160+, Uc.283+A and Uc.346+ methylation in the colorectal cancer cell lines HCT-116 and DKO, normal colon (NC) and normal lymphocytes (NL). Unmethylated (U) or methylated (M) sequences. In vitro methylated DNA (IVD) is shown as a positive control for methylated sequences. The locations of the methylation-specific PCR primers are indicated in panel ‘a' by gray arrows.