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(A) Representative photomicrograph showing dual retrograde labeling of neurons in the guinea pig nucleus ambiguus (nAmb) following administration of different fluorophore-conjugated cholera toxin B (CTb) subunits to the trachea (red) and esophagus (green). (B) Bar chart displaying the cumulative number of single or double retrogradely labeled neurons in the nAmb (n = 4 experiments). The scale bar in (A) represents 50 μm.
