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Kinetics of IS and contact formation by a CTL clone. A CER43 cell on a bilayer containing ICAM-1 and agonist MHCp complexes (GL9). (a) Interference reflection microscopy images showing contact area development (darker areas represent closer contact). (b) Fluorescence images show IS formation. Cy5–ICAM-1–GPI is red and Alexa Fluor 488–HLA-A2–GL9 is green. Scale bar: 5 μm.
