Figure 5.

Three-dimensional distribution of granules and Golgi complexes in CTLs forming ISs or ring junctions. Tilted three-dimensional views of CTL CER43 cells stained with LysoTracker red (granules; red) and BODIPY ceramide (Golgi; green). Cells were layered on bilayer containing human Cy5–ICAM-1–GPI (blue) only (a and b), where they form a ring junction, or on a bilayer containing human Cy5–ICAM-1–GPI (blue) and unstained agonist MHCp complexes (c and d), where they form an IS. Two examples are provided for each substrate to illustrate the range of patterns that were typical of many more cells scored and summarized in Table 1. All images were acquired within an hour of contact with the bilayer. The z-stack interval is 1 μm with the bilayer level defined at zero.