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. 2004 Jan 1;113(1):28–37. doi: 10.1172/JCI200419491

Figure 1.

Figure 1

Conditional deletion of Stat-3 in respiratory epithelium. (a) The human SP-C (hSP-C) promoter was used to express the reverse tetracycline transactivator (rtTA). In the presence of doxycycline (Dox), rtTA binds to the (tetO)7 promoter, activating transcription of Cre-recombinase. The loxP sites were inserted in introns 20 and 21 of the Stat-3 gene (Stat-3flx), deleting exon 21 after recombination, to produce the Stat-3Δ locus. (b) STAT-3 RNA was assessed in purified alveolar type II cells. RNA was extracted from primary cultures of alveolar type II cells isolated from Stat-3Δ/Δ and control mice. RNA from control (n = 2) or Stat-3Δ/Δ (n = 2 or 4) were pooled and analyzed by real-time RT-PCR. Data were standardized to β-actin RNA and normalized to control. In the Stat-3Δ/Δ cells, STAT-3 mRNA was less than 10% of control values.