Figure 5.
STAT-3 immunohistochemistry after exposure to 95% oxygen. Lungs from control (a) and Stat-3Δ/Δ (b) mice were stained for Stat-3 after 72 hours of exposure to 95% O2. Alveolar type II cells, bronchiolar epithelial cells, and alveolar macrophages were stained in controls. Stat-3 was detected in inflammatory cells (filled arrows), vascular and stromal cells, but not in epithelial cells (arrowheads), in Stat-3Δ/Δ mice. Bronchiolar epithelial cell necrosis was prominent (open arrows). Original magnification ×10. Magnification for inserted enlarged photomicrographs ×40.