TABLE 1.
Expression of ICAM-1 at transcriptional levela
| Method | No. of patients | Fold change |
|
|---|---|---|---|
| AOM/carri. | AOM/conval. | ||
| Microarray | 1 | 2.52 | 10.21 |
| qRT-PCR | 6 | 2.76 | 1.13 |
a
Total RNA was extracted from peripheral blood mononuclear cells from the same child at three time points: at the time of AOM caused by S. pneumoniae, before the S. pneumoniae infection (carriage [carri.]), and after the infection during the convalescent (conval.) stage. Microarray analysis and qRT-PCR were performed as described in Materials and Methods. Data were analyzed after normalization (see Materials and Methods). The data for qRT-PCR are the mean of 6 children.