FIG. 1.

Schematic representation of the full-length and truncated gene products (SpaC427, the N-terminal-half domain; and SpaC253, the C-terminal domain). The numbers indicate the first and last amino acid (aa) residues of each construct. The 29-amino-acid signal sequence, immunoprotective domain (the α-helical domain), proline-rich region, and 20-amino-acid repeat domain are indicated by solid, open, stippled, and numbered boxes, respectively. Arrows indicate the position and orientation of primer sites. F, forward; R, reverse.