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(A to D) Coomassie blue-stained SDS-12% PAGE gels (A) and immunoblot analysis of alkaline extracts of E. rhusiopathiae strains (native Spa proteins) probed with polyclonal antisera from mice immunized with rSpaC664 protein (B), rSpaC427 protein (C), and rSpaC253 protein (D). The alkaline extracts of E. rhusiopathiae strains Fujisawa expressing SpaA (lane 1), Dolphin E-1 expressing SpaB (lane 2), and 715 expressing SpaC (lane 3). Numbers on the left are molecular masses in kilodaltons.
