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ELISA detection of rSpaA, rSpaB, rSpaC664, rSpaC427, and rSpaC253 antibody titers in the sera of pigs vaccinated with SpaC427 (n = 6) and of the nonimmunized control pigs (n = 3). Antibody titers are expressed as the average titer ± the standard deviation of the average of six or three pigs. Pigs were immunized and boosted 3 weeks apart with 100 μg of rSpac427. Serum samples were collected just before challenge.
