TABLE 2.
Active immunization of mice against challenge with various serovar strains of E. rhusiopathiae
| Immunizationa | Challenge strain (serovar, Spa protein expressed)b |
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|---|---|---|---|---|---|---|---|---|---|---|
| 715 (18, SpaA) |
2017 (19, SpaB) |
Dolphin E (6, SpaB) |
ATCC 19414T (2, SpaA) |
Fujisawa (1a, SpaA) |
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| No. alive/ total no. | P | No. alive/ total no. | P | No. alive/ total no. | P | No. alive/ total no. | P | No. alive/ total no. | P | |
| rSpaC664 | 8/10 | 0.0004 | 9/10 | 0.0001 | 7/10 | 0.0015 | 8/10 | 0.0004 | 7/10 | 0.0015 |
| rSpaC427 | 10/10 | 0.0000 | 10/10 | 0.0000 | 9/10 | 0.0001 | 10/10 | 0.0000 | 10/10 | 0.0000 |
| rSpaC253 | 0/10 | 1.0000 | 0/10 | 1.0000 | 1/10 | 0.3049 | 0/10 | 1.0000 | 0/10 | 1.0000 |
| Control | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | |||||
a
Mice were immunized and boosted 3 weeks apart with 40 μg and challenged 2 weeks after the boost.
b
Data are presented as the number of mice alive/the total number of animals tested. The survival of mice was monitored for 14 days after challenge with strains of E. rhusiopathiae. P values are based on a comparison of alive/dead ratios for immunized mice with those for control mice, as calculated by the Fisher exact test.