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Comparison of different elution buffers for the removal of PCR inhibitors in aerobically digested biosolids
| Type of elution buffer | Template amt (pg) | No. of positive PCRs/total PCRs (by sample dilution) |
||
|---|---|---|---|---|
| 1:1 | 1:10 | 1:100 | ||
| Beef extract | 1,600 | 0/3 | 0/3 | 3/3 |
| 160 | 0/3 | 0/3 | 3/3 | |
| 16 | 0/3 | 0/3 | 3/3 | |
| Glycine | 1,600 | 0/3 | 1/3 | 3/3 |
| 160 | 0/3 | 1/3 | 3/3 | |
| 16 | 0/3 | 1/3 | 3/3 | |
