SDS-PAGE (A) and Western blot (B) profiles showing expression of anti-SEB ScFv in soluble form. (A) Lane 1, molecular mass markers (66, 45, 29, 20, and 14.2 kDa); lane 2, uninduced culture of 4PCL2-infected HB2151 cells; lanes 3 and 4, induced culture and periplasmic extract of 4PCL2-infected HB2151 cells, respectively, showing the anti-SEB ScFv band at ∼29 kDa. (B) Lane 1, molecular mass markers (97.4, 66.2, 45, 31, 26.6, and 14.4 kDa); lane 2, uninduced culture of 4PCL2-infected HB2151 cells; lanes 3 and 4, induced culture and periplasmic extract of 4PCL2-infected HB2151 cells, respectively, showing the anti-SEB ScFv band at 29 kDa.