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ELISA (A) and Western blot (B) showing binding of anti-SEB ScFv with SEB antigen. ELISA was performed with different dilutions of soluble anti-SEB ScFv antibody. Periplasmic extract of untransformed HB2151 cells was used as a negative control (OD, 0.0945). (B) Lane 1, prestained molecular mass markers (70, 55, 35, 27, and 15 kDa); lane 2, r-SEB band at 30.5 kDa showing binding with anti-SEB ScFv. The bound anti-SEB ScFv antibody was detected using anti-E-tag HRP-conjugated antibody.
