TABLE 1.
E. coli strains used in glycation studies
| Strain | Genotypea |
|---|---|
| Parental strains | |
| ZK126 | W3110 ΔlacU169tna-2 |
| ZK1142 | ZK126 Nalr |
| ZK1143 | ZK126 Strr |
| GloA− strains | |
| SF2671 | ZK126 gloA::Camr |
| SF2672 | ZK126 gloA::Kanr |
| SF2673 | SF2671 gloA::FRT |
| SF2674 | ZK1142 gloA::Camr |
| SF2675 | SF2674 gloA::FRT |
| SF2676 | ZK1143 gloA::Camr |
| SF2677 | SF2676 gloA::FRT |
| GloB− strains | |
| SF2678 | ZK126 gloB::Camr |
| SF2679 | SF2678 gloB::FRT |
| SF2680 | ZK1142 gloB::Camr |
| SF2681 | SF2680 gloB::FRT |
| SF2682 | ZK1143 gloB::Camr |
| SF2683 | SF2682 gloB::FRT |
| GloA− GloB− strains | |
| SF2684 | ZK126 gloA::FRT gloB::Kanr |
| SF2685 | SF2684 gloA::FRT gloB::FRT |
| SF2686 | ZK1142 gloA::FRT gloB::Kanr |
| SF2687 | SF2686 gloA::FRT gloB::FRT |
| SF2688 | ZK1143 gloA::FRT gloB::Kanr |
| SF2689 | SF2688 gloA::FRT gloB::FRT |
a
Abbreviations: Nalr, nalidixic acid resistant; Strr, streptomycin resistant; Camr, chloramphenicol resistant; Kanr, kanamycin resistant; FRT, chloramphenicol or kanamycin resistance cassette removed with FLP recombinase, leaving a single FRT site in its place (see reference 10).