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Comparison between rNVLP binding to PGM and RE was assayed by ELISA. (A) The involvement of sugar and protein moieties in rNVLP binding was determined by treatment of PGM or RE with KIO4 or after being boiled. (B) Competition assay in which rNVLP were preincubated with the listed dilution amount of RE prior to incubation on PGM-coated plates. (C) rNVLP were preincubated with the listed amount of PGM prior to incubation on RE-coated plates. Error bars represent SD.
