FIG. 1.

Reverse line blotting results. (A) Hybridization signal obtained with different controls. Lanes: 1, 10² GE B. taylorii CIP 107028; 2, 10² plasmid copies with the insert ITS (PCITS) Bartonella spp. from a Spanish mole (15); 3, 10² PCITS from GT 8; 4, 10² PCITS from GT 9; 5, 10² PCITS from GT 21; 6, 10² PCITS from GT 22; 7, 10² PCITS from GT 6; 8, 10² PCITS from GT 14; 9, 10² PCITS from GT 16; 10, 10² PCITS from GT 17; 11, 10² PCITS from GT 18; 12, 10² GE B. elizabethae CIP 103761; 13, 10² GE B. doshiae CIP 107026; 14, 10² PCITS from GT 19; 15, 10² GE B. grahamii CIP 107024;16, 10² PCITS from GT 12; 17, 10² PCITS from GT 13; 18, 10² PCITS from GT 20; 19, 10² PCITS from GT 10; 20, 10² GE B. tribocorum CIP 105476; 21, 10² GE B. phoceensis CIP 107707; 22, 10² GE B. rattimassiliensis CIP 107705; 23, 10² GE B. vinsonii subsp. arupensis CIP 106848; 24, 10² GE B. vinsonii subsp. vinsonii CIP 103738; 25, 10² GE B. alsatica CIP 105477; 26, 10² GE B. quintana CIP 103739; 27, 10² GE B. bacilliformis CIP: 77.27; 28, 10² GE B. henselae CIP 103737; 29, 10² GE B. clarridgeiae CIP 104772; 30, 10² GE B. koehlerae CIP107025; 31, 10² PCITS B. rochalimae from a Spanish fox (15); 32, 10² PCITS Bartonella spp. from a Spanish badger (15); 33, 10² GE B. vinsonii subsp. berkhoffii CIP 104960; 34, 10² GE B. bovis CIP 106692; 35, 10² GE B. schoenbuchensis CIP 107819; 36, 10² GE B. capreoli CIP 106691; 37, 10² GE B. chomelii CIP 107869; 38, 10² GE B. birtlesii CIP 106294; 39, 10² PCITS Ochrobactrum sample PVR70-01Sh; 40, negative PCR control; 41, 10² PCITS from GT 2; 42, 10² PCITS from GT 4. Lanes where clones were tested have no hybridization signals with the S-BART16S probe because the control only contains the ITS target. (B) Examples of hybridization reactions with several small mammal samples.