TABLE 1.
Primers and probes used in the studya
| Target | Organism | Primer | Probe | Sequenceb | Concn (μM)c |
|---|---|---|---|---|---|
| ITS | Bartonella spp. | Bart/16-23F | 5′-bio-TTG ATA AGC GTG AGG TCG GAG G | 0.4 | |
| Bart/16-23R | 5′-bio-CAA AGC AGG TGC TCT CCC AG | 0.4 | |||
| B. taylorii | S-TAY2 | 5′-a-TAT CCA TTT CSC TTA GGC A | 3.2 | ||
| Bartonella spp.d | S-TALP | 5′-a-CAG TCC CTT TAG GTC CAT TTA ATC | 3.2 | ||
| Bartonella GT 8 | S-MUS1 | 5′-a-GTC TAT TGG ATT TAA GTG TTG | 0.4 | ||
| Bartonella GT 9 | S-MUS2 | 5′-a-GGT CCG TTT GTT AAG TGT TGG | 3.2 | ||
| Bartonella GT 21 | S-MUS3b | 5′-a-CTT TGC GAG ACT TTT TCA CTC C | 0.4 | ||
| Bartonella GT 22 | S-TOPO15b | 5′-a-CTT AAC TTG TTG AAG GCT CCC | 3.2 | ||
| Bartonella GT 6 | S-CE1MM3b | 5′-a-GAA CTC CAT ATA AAA GGC TTT AAA TAT TG | 3.2 | ||
| Bartonella GT 14-15 | S-OR3MM1 | 5′-a-AAT CAA ATT TAA GCA ATA CAA ATC | 3.2 | ||
| Bartonella GT 16 | S-R2Eb | 5′-a-AGT GCC TTT GTT AGA GAA TAC C | 3.2 | ||
| Bartonella GT 17 | S-OR3MM3 | 5′-a-AAG AAT AAA AGT CAA AAT AAT ATT G | 1.6 | ||
| Bartonella GT 18 | S-CAT9b | 5′-a-GTG TAT TAA ACG TAT CAA AGC CTC | 3.2 | ||
| B. elizabethae | S-ELIZ | 5′-a-TAA GTT CCC TTC AAG AGG ATA | 3.2 | ||
| B. doshiae | S-DOSH | 5′-a-TTT GAA CCT TCT CTC TTT AT | 3.2 | ||
| Bartonella GT 19 | S-APO38 | 5′-a- CCT TTT CTC CTT TTT AGG GGC | 3.2 | ||
| B. grahamii | S-GRAH2 | 5′-a-ATT CAA GTT GAT GAA TTT GGT TAT | 0.4 | ||
| Bartonella GT 12 | S-GU1MM1 | 5′-a- TCA AAT TGG TGA ATC TGG TTA T | 3.2 | ||
| Bartonella GT 13 | S-CAT6 | 5′-a- TAA AGA GAA GTT TGT CCA AGA G | 3.2 | ||
| Bartonella GT 20 | S-APS48 | 5′-a-ATC ACT GAA AGT TGC TCT GAG T | 1.6 | ||
| Bartonella GT 10 | S-R24Sh | 5′-a-GCT TTT CTG TTT GCC TGA GGT C | 3.2 | ||
| B. tribocorum | S-TRIB | 5′-a-TTC TAT TAA GTT TGT CAA AGG G | 0.4 | ||
| B. phoceensis | S-PHO | 5′-a-GAG AGA CGC TTT TCC CTT TGG | 1.6 | ||
| B. rattimassiliensis | S-RAT | 5′-a-CGG TGT TTT GAG GCA AAG TGC | 1.6 | ||
| B. vinsonii subsp. arupensis | S-VIN-A1 | 5′-a-ACTTGTTGGAATTGCTTAACC | 3.2 | ||
| B. vinsonii subsp. vinsonii | S-VIN-A2 | 5′-a-ATGAAAATATTGAGAGATTTG | 3.2 | ||
| B. alsatica | S-ALS | 5′-a-GCT GGT GAA ACT TGC TTA TA | 6.4 | ||
| B. quintana | S-QUIN | 5′-a-CGC TTA TCC ATT TGG TTT AA | 3.2 | ||
| B. bacilliformis | S-BACI | 5′-a-CCT ATG ATT GAT TTC TAG GC | 0.4 | ||
| B. henselae | S-HENS | 5′-a-ATC GGT TCA ATC ATA TCG CTT T | 3.2 | ||
| B. clarridgeiae | S-CLARR2 | 5′-a-ACG ATG CTA AAA GTT GCT ATA TTG | 3.2 | ||
| B. koehlerae | S-KOE | 5′-a-TTA AAT TAT ATC ACT TTG GGT CAT ACG | 0.4 | ||
| B. rochalimae | S-ZOR | 5′-a-AAC AGG GAA AAG AGC AGG CCA | 3.2 | ||
| Bartonella spp. detected in badgerd | S-TEJ | 5′-a-GAT GTT TTG TAA AAG TGC GTC G | 3.2 | ||
| B. vinsonii subsp. berkhoffii | S-VIN-B | 5′-a-TTT CGG ACA CTA TTG ATA AA | 3.2 | ||
| B. bovis | S-BOV2 | 5′-a-CGT TTT GAT AGT CTT TTG TGT TGC | 0.4 | ||
| B. chomelii, B. schoenbuchensis, B. capreoli, and B. birtlesii | S-CHOSCA | 5′-a-TTA TGA TTG CTG ATA AGT TTG CTG | 3.2 | ||
| O. grignonense | S-GRIGNO | 5′-a- GCT TTG ATA AAT GTG ATA AGG | 1.6 | ||
| 16S rRNA | Bartonella spp. | 16S-R | 5′-bio-GCC YCC TTG CGG TTA GCA CAG CA | 1 | |
| P24Emod | 5′-bio-CCT TCA GTT MGG CTG GAT C | 1 | |||
| S-BART16S | 5′-a-CTC GCC CTT AGT TGC CAG CAT T | 3.2 | |||
| TCH synthase | Cannabis sativa | CI-F | 5′-bio-ATG ATG CTG AGG GTA TGT CCT AC | 1 | |
| CI-R | 5′-bio-GTT TTC TCC TCC ACC ACC ACG | 1 | |||
| S-CI2 | 5′-a-GTG GAC ACT TTA GTG GAG GAG G | 3.2 | |||
| gltA | Bartonella spp. | GLTAF2 | 5′-GCT TTK CTG TTC CDT GTG AAG | 1 | |
| GLTAR2 | 5′-GCA AAA AGA ACA GTA AAC ATT TC | 1 | |||
| GLTAF1 | 5′-AAA ATG CTA CAA GAA ATH GG | 1 | |||
| GLTAR1 | 5′-AGC TTT TAA TGT AAT DCC DG | 1 |
a
The probes and primers in boldface were designed for this study. The rest of the oligonucleotides have been described previously (15).
b
Oligonucleotide modifications at the 5′ end. bio, biotin; a, amino link.
c
That is, the concentration of the oligonucleotides used in the assays.
d
Garcia-Esteban et al. (15).