FIG. 1.

Effects of cold shock and cold acclimation at 5°C on the growth of Lactococcus piscium CNCM I-4031 in Elliker broth. Cells were harvested from standard cultures at 26°C at mid-exponential phase and grown at 5°C (×), at 26°C without cold shock (•), and at 26°C after 1 h at 0°C (○), 2 h at 0°C (□), 1 h at 5°C (⋄), and 2 h at 5°C (▵). Standard deviation values were lower than 0.03 and are not indicated on the graph (n = 9; three replicates of three independent cultures).