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Immunoblotting of E. coli inclusion bodies and total extracts of A. bisporigera with anti-AbPOPB antibody. Lanes: 1, BL21 empty vector (PET21) control; 2, inclusion body (1 μl = 0.05 μg) of an E. coli BL21 line expressing AbPOPB; 3 through 6, total soluble protein from A. bisporigera loaded at 24 μg, 47 μg, 94 μg, and 141 μg, respectively. All lanes were from the same gel, but some lanes between lanes 1 and 2 were removed. Size indicators (left) are in kDa.
