Fig. 2.

Complemented T. thermophila strains show normal growth and express similar levels of tubulin. (A) T. thermophila wild-type (WT) and mutant strains grow with similar kinetics at 30°C in the absence of oryzalin. (B) Immunoblot analysis of cell lysates equivalent to ∼9,750 cells per well indicated that the three T. thermophila strains (the wild-type complemented strain and strains expressing tubulin with the L136F or V252L point mutation) express essentially equivalent concentrations of tubulin per cell (30 pg). This blot was probed with the anti-α-tubulin monoclonal antibody 1-5-2.