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. 2010 Dec;9(12):1825–1834. doi: 10.1128/EC.00140-10

Fig. 3.

Fig. 3.

(A) Growth of complemented strains in 0, 7.5, and 15 μM oryzalin. (A) T. thermophila cells expressing the L136F ATU1 transgene (top panel) and T. thermophila cells expressing the I252L ATU1 transgene (bottom panel). (B to D) Immunofluorescence of T. thermophila cells cultured in oryzalin. Wild-type T. thermophila cells grown in 7.5 μM oryzalin lose most or all cilia but retain underlying cortical microtubules (B), whereas T. thermophila cells expressing the L136F (C) or I252L (D) tubulin mutations retain cilia.