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. 2010 Dec;9(12):1825–1834. doi: 10.1128/EC.00140-10

Fig. 6.

Fig. 6.

Mutant tubulins show altered assembly properties. (A) Polymerization of 0.7 mg/ml (7 μM) purified T. thermophila tubulin was followed at 351 nm in the presence (gray) or absence (black) of 0 or 5 μM oryzalin. Wild-type T. thermophila tubulin assembles normally in the absence of oryzalin but fails to polymerize in 5 μM oryzalin. L136F T. thermophila tubulin and I252L T. thermophila tubulin both assemble in 5 μM oryzalin. (B) Determination of Cc values for wild-type, L136F, and I252L tubulins. Wild-type T. thermophila tubulin has a Cc of 0.55 ± 0.09 mg/ml. The Cc for L136F tubulin is dramatically decreased to 0.25 ± 0.07 mg/ml, whereas I252L tubulin behaves similarly to wild-type tubulin with a Cc value of 0.56 ± 0.06 mg/ml.