Fig. 6.
Cbf1p regulation of INO1 expression depends on Ino2p-Ino4p-binding sites in the INO1 promoter. (A) WT and isogenic cbf1Δ knockout strains were transformed with an INO1-1200-lacZ plasmid (wild-type INO1 promoter) or an INO1-100-lacZ plasmid (UASINO-deleted INO1 promoter). (B) WT and isogenic cbf1Δ knockout strains were transformed with pJH330 containing mutations of the E1 box, the E2 box, or both the E1 and E2 boxes. These transformants were assayed for β-galactosidase activity as described above. The data represent means and standard errors of the means from at least three different experiments.