Fig. 8.

Sporulation ability of the cam1 mutant harboring mutations in EF-hand motifs. The wild-type cam1⁺ and respective cam1 mutant genes were integrated at the leu1 locus on chromosome II of AI509. Strains used were AI510 (cam1⁺), AI512 (cam1-22), AI518 (cam1-22, 117), AI520 (cam1-EF1), AI522 (cam1-EF2), AI524 (cam1-EF3), and AI526 (cam1-EF4). Strains were cultured on MEA medium for 2 days, and their sporulation efficiencies were measured. The percentage of asci, classified according to the number of spores per ascus, is presented.