Table 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pAL-KS | ars1 LEU2-based vector | 31 |
| pBR(leu1) | leu1+ in pBR322 | 22 |
| pREP1 | ars1 LEU+-based vector carrying a thiamine-repressible nmt1 promoter | 16 |
| pIL2 | LEU2-based integration vector | 20 |
| pIA | ade6+-based integration vector | K. Tamai |
| pAL(cam1) | cam1+ in pAL-KS | This study |
| pAL(gfp-cam1) | gfp-cam1+ in pAL-KS | This study |
| pIA(gfp-cam1) | gfp-cam1+ in pIA1 | This study |
| pREP1(ade6) | ade6+-based expression vector | T. Nakamura |
| pREP1(ade6)gfp-cam1+ | gfp-cam1+ in pREP1(ade6) | This study |
| pREP1(ade6)gfp-cam1-22 | gfp-cam1-22 in pREP1(ade6) | This study |
| pREP1(ade6)gfp-cam1-22,117 | gfp-cam1-22,117 in pREP1(ade6) | This study |
| pREP1(ade6)gfp-cam1-EF2 | gfp-cam1-EF2 in pREP1(ade6) | This study |
| pREP1(ade6)gfp-cam1-EF3 | gfp-cam1-EF3 in pREP1(ade6) | This study |
| pBR322(spo13-gfp) | spo13+-gfp in pBR(leu1) | Y. Nakase |
| pBR322(spo2-gfp) | spo2+-gfp in pBR(leu1) | Y. Nakase |
| pREP1(spo2) | spo2+ in pREP1 | Y. Nakase |
| pREP1(spo13) | spo13+ in pREP1 | Y. Nakase |
| pREP1(spo15) | spo15+ in pREP1 | T. Nakamura |
| pIA(gfp-psy1) | gfp-psy1+ in pIA | 14 |
| pBR(gfp-cam1+) | gfp-cam1+ in pBR(leu1) | This study |
| pBR(cam1+) | cam1+ in pBR(leu1) | This study |
| pBR(cam1-22) | cam1-22 in pBR(leu1) | This study |
| pBR(cam1-22,117) | cam1-22,117 in pBR(leu1) | This study |
| pBR(cam1-EF1) | cam1-EF1 in pBR(leu1) | This study |
| pBR(cam1-EF2) | cam1-EF2 in pBR(leu1) | This study |
| pBR(cam1-EF3) | cam1-EF3 in pBR(leu1) | This study |
| pBR(cam1-EF4) | cam1-EF4 in pBR(leu1) | This study |