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. 2010 Dec;9(12):1835–1844. doi: 10.1128/EC.00143-10

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Fig. 5. — PAH3 and PAH4 are required to reestablish SPO13 repression in meiotic cells. Isogenic wild-type (RSY877), sin3Δ (RSY1028), pahΔ1 (RSY1029), pahΔ2 (RSY1030), pahΔ3 (RSY1031), or pahΔ4 (RSY1032) strains were induced to enter meiosis, and time points were taken as indicated. Northern blots of total RNA were probed with SPO13 (black arrowheads) and then stripped and probed sequentially with probes directed toward SPS4 (gray arrowheads) and ENO1 (white arrowheads). (B) Quantitation of SPO13 signals in panel A following standardization with ENO1. The time point exhibiting the maximum signal from each time course was set at 100%. (C) The percentage of maximum induction of the 12-h signal from panel B is shown for the SIN3 alleles indicated. (D) Meiotic progression in the wild type (RSY877) and pahΔ4 mutant (RSY1032) was determined by monitoring nuclear divisions using DAPI analysis. The percentage of each population executing at least one nuclear division is shown.