Sin3p displays expanded meiotic regulatory targets. (A) S1 nuclease protection assay monitoring IME1, SPO13, and SPS2 mRNA levels in wild-type (RSY887) and sin3 (RSY1028) cultures in extended meiotic time courses. Arrows indicate the protected riboprobe for each mRNA. (B) Quantitative PCR was utilized to monitor mRNA levels of the indicated genes at either 12 h (top) or 18 h (bottom) in wild-type (RSY877), sin3Δ (RSY1028), pahΔ3 (RSY1031), or pahΔ4 (RSY1032) strains. The graphs are expressed as fold increase over wild-type mRNA levels at a given time point. Note the split axes for the 18-h time points.