Figure 11.

Effect of oxidized PAPC vesicles on the binding of NO₂-LDL to hMDM and MPM. [¹²⁵I]LDL was modified as described for the complete system in Figure 2. [¹²⁵I]NO₂-LDL (5 μg/mL) was then incubated with (a) MPM or with (b) hMDM for 3 hours at 4°C either in the absence (NA) or presence of the indicated additions. Cellular binding of lipoproteins was subsequently determined as described in Methods. The concentrations of competitors used were 200 μg protein/mL for lipoproteins, 20 μg lipid/mL for vesicles, and 20 μg/mL for antibody. NO₂-LDL, LDL modified by the MPO-H₂O₂-NO₂^– system as in the complete system of Figure 2a; –NO₂LDL, LDL modified by the MPO-H₂O₂ system but in the absence of NO₂^–; NO₂-PAPC, PAPC vesicles modified by the complete MPO-H₂O₂-NO₂^– system; –NO₂ PAPC, PAPC modified by the MPO-H₂O₂ system but in the absence of NO₂^–. Data represent the mean ± SD of triplicate determinations of a representative experiment performed at least 3 times. ^AP < 0.001 for comparison versus control (NA).