Figure 6.

Time dependence of LDL recognition by macrophages from wild-type mice and CD36-null mice after exposure to the MPO-H₂O₂-NO₂^– system. [¹²⁵I]LDL was incubated with isolated human MPO, a H₂O₂-generating system (GGOx), and NO₂^– in sodium phosphate buffer (50 mM, pH 7.0) supplemented with DTPA (100 μM) for the indicated times as described in Methods. Binding (a) at 4°C and degradation (b) at 37°C of the modified lipoprotein by macrophages from wild-type mice (CD36^+/+, filled squares) and CD36-null mice (CD36^–/–, filled circles) were then determined as described in Methods. Data represent the mean ± SD for triplicate determinations of a representative experiment performed at least 3 times.