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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Cell Cycle. 2010 Apr 1;9(7):1411–1420. doi: 10.4161/cc.9.7.11255

Figure 4.

Figure 4

Inhibition of MDM2 is sufficient to induce apoptosis in TGCT cells. (A) Cells were treated with 5 µM Nutlin for 18 hrs, fixed and stained with propidium iodide, and analyzed by FACS to detect the Sub-2N apoptotic cells. (B) Representative photographs of TGCT cells undergoing apoptosis after Nutlin treatment, compared to growth arrest response by HCT116 cells. (C) Cells were treated with 5 µM Nutlin for 12 hrs and labeled with methyl-3H-thymidine for 1 hr. The rate of DNA synthesis was determined by scintillation counting.