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. Author manuscript; available in PMC: 2012 Jan 20.
Published in final edited form as: Virology. 2010 Nov 9;409(2):299–307. doi: 10.1016/j.virol.2010.10.022

Fig. 2.

Fig. 2

A. GST-pulldowns of FLAG-cdk8 from cell lysates with GST, GST-rv-cyclin (rv-cyclin), and GST-cyclin C (cyclinC). GST-CTD served as in vitro substrate in kinase assays of bound cdk8 (top panel: autoradiograph of western blot of kinase reactions (32P-CTD)) followed by sequential antibody detection of input GST-CTD substrate (αCTD; anti-RNAPII CTD) and pulled down cdk8 (αcdk8). B. Ni Sepharose-His-cdk8 pulldown of soluble GST, GST-rv-cyclin (rv-cyclin), or GST-cyclin C (cyclinC) probed with anti-GST antibody (αGST). The blot was reprobed with anti-polyHis (αHis) to detect input His-cdk8. Aliquots of input GSTs were run separately (bottom panel). GST-rv-cyclin and GST-cyclin C are of similar molecular weight (64 KDa vs. 60 KDa); the GST panels show signal for proteins at 27 KDa (GST).