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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: J Immunol. 2010 Nov 12;185(12):7480–7486. doi: 10.4049/jimmunol.1002771

FIGURE 2.

FIGURE 2

Accessory cell-NK cell contact is necessary for NK cell activation in vitro. Purified NK cells (DX5+CD3−) were co-cultured with macrophages either mixed together (Mixed) or separated by a transwell system (Transwell), followed by stimulation with Ad-LacZ for 18 h (Ad), or left unstimulated (Medium). Brefeldin A was added into each well and incubated for additional 4 h. (A) DX5+CD3− NK cells were analyzed for the expression of CD69. MFI of CD69 staining is indicated. (B-C) DX5+CD3− NK cells were analyzed for intracellular IFN-γ and granzyme B production. FACS plots of IFN-γ and granzyme B production with the percentage of IFN-γ and granzyme B positive cells indicated (B). The mean percentage ± SD of IFN-γ or granzyme B positive cells is indicated (n = 5 per group) (C). The data shown are representative of three independent experiments.