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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: J Immunol. 2010 Nov 12;185(12):7480–7486. doi: 10.4049/jimmunol.1002771

FIGURE 5.

FIGURE 5

NKG2D is required for NK cell activation upon adenoviral infection in vivo. (A-B) C57BL/6 mice were treated with 100 μg of anti-NKG2D mAb (+Anti-NKG2D) or the control rat IgG (+Rat IgG) 24 h and 6 h before intravenous injection of Ad-lacZ (Ad). Some mice were left uninfected as controls (Naïve). 24 h after infection, splenocytes were harvested and restimulated with PMA/Inomycin in the presence of Brefeldin A for 4 h. Intracellular IFN-γ and granzyme B was determined by flow cytometry gated on DX5+CD3− NK cells. FACS plots of IFN-γ and granzyme B production with the percentage of IFN-γ and granzyme B positive cells indicated (A). The mean percentage ± SD of IFN-γ or granzyme B positive cells is indicated (n = 4 per group) (B). The data shown are representative of three independent experiments.