Table 1.
Oligonucleotide forward and reverse primer sequences (5′-3′), PCR product length, linearity, efficiency, and % intra-assay variation of real-time RT-PCR reactions
| Gene | Primer Sequence | Product Size, bp | Accession No. | r2 | E | % Variation |
|---|---|---|---|---|---|---|
| SAA1 | F CAACTACAGGGGTGCAGACA | 241 | XM_867996 | 0.998 | 1.03 | ± 3.9 |
| R AGCAGGTCTGAAGTGGTTGG | ||||||
| GPX3 | F ACACGAGTACAGGGCCACAT | 201 | NM_174077 | 0.997 | 1.04 | ± 5.5 |
| R GGTAGGGTGAGGTCCTGGTT | ||||||
| FOXA3 | F GCCATGTCATCCCTGTCTCT | 176 | NM_001033119 | 0.996 | 1.08 | ± 0.1 |
| R CCGGCAAGAACCAGACTTTA | ||||||
| ANGPT4 | F GAATTTCCAACGGAACTGGA | 113 | EU599227 | 0.98 | 1.28 | ± 21.0 |
| R GAGAGTAGGTTGCCCTGCTG | ||||||
| ADIPOR2 | F CCAGAGGAGCCCCACAGT | 117 | BT025411 | 1.0 | 0.89 | ± 6.0 |
| R GCCATCTCGCGTCTTCTC | ||||||
| CD36 | F CAGCATCAGCAAGGCATTA | 115 | BC103112 | 0.99 | 1.23 | ± 1.5 |
| R CAGAAGAGCTGTAGAACGTGGA | ||||||
| CLDN1 | F CCATTGCTAAAAATGACTTAGCC | 120 | BT021861 | 0.999 | 1.03 | ± 0.1 |
| R AAGGAATGCTATCTCCCCTCA | ||||||
| CPT1A | F TCCTGGTGGGCTACCAATTA | 181 | FJ415874 | 0.999 | 0.99 | ± 2.6 |
| R TGCGTCTGTAAAGCAGGATG | ||||||
| CPT1B | F GCGCTTTGGGAACCAGAT | 128 | NM_001034349 | 0.998 | 1.08 | ±16.5 |
| R CCCCCTCCTCCACTCTGT | ||||||
| HP | F TCGTCGTTCACGACAAGG | 129 | BC109668 | 0.995 | 1.13 | ± 0.1 |
| R TTTTCCGAACCCAGTCCA | ||||||
| IL8 | F AAAAGACACAAACAGAAAGACCTC | 140 | AF232704 | 0.99 | 1.06 | ± 0.0 |
| R GCATCTCAAAAGTAGGACTTCCA | ||||||
| TNFα | F CATCCTGTCTGCCATCAAGA | 147 | EU276079 | 0.995 | 0.93 | ± 19.4 |
| R TAGTCCGGCAGGTTGATCTC | ||||||
| ACADVL | F ATCGTCCACCAGGAACTGAG | 130 | BT030546 | 0.999 | 1.05 | ± 5.2 |
| R GCTGCAGCAGAAACTGTTCA | ||||||
| PPARA | F TTGTGGCTGCTATCATTTGC | 135 | NM_001034036 | 0.998 | 1.0 | ± 2.9 |
| R AGAGGAAGACGTCGTCAGGA | ||||||
| PPARG | F GTGAAGCCCATTGAGGACAT | 149 | NM_181024 | 0.999 | 0.95 | ± 2.4 |
| R AGCTGCACGTGTTCTGTCAC | ||||||
| PPARD | F CACTCTCACTGCTGGACCAA | 194 | NM_001083636 | 0.99 | 0.98 | ± 2.9 |
| R GCAGATCCGCTCACATTTCT | ||||||
| RxRG | F ATGAAGATATGCCCGTGGAG | 126 | NM_001075408 | 0.991 | 1.01 | ± 11.8 |
| R CAGCGTGGCATATATTGGTG | ||||||
| HSP90 | F GGCGCTGATATCTCCATGAT | 119 | BC151818 | 0.99 | 1.07 | ± 0.9 |
| R GACTCCCAGGCATACTGCTC | ||||||
| FABP1 | F GGAGTTCATGACTGGGGAGA | 135 | NM_175817 | 0.99 | 1.22 | ± 12.7 |
| R CCCTTCGTCATGGTACTGGT | ||||||
| FADS2 | F CTGACTGGTGATGGACCTGA | 127 | NM_001083444 | 0.99 | 1.09 | ± 5.6 |
| R TCCCTATGGATCCAGTCTGC | ||||||
| GSTA4 | F TTCATGCCATAGGACAGCAG | 111 | BT020960 | 0.9997 | 1.02 | ± 4.3 |
| R ACGACAGAGCTGGATCACG |
r2, Linearity; E, efficiency; F, forward; R, reverse.